Composite

Part:BBa_K4475009

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-09-29)


CTB3 Inducible Protein Generator Yeast

Construct designed for inducible expression of the fungal enzyme CTB3 in saccharomyces cerevisiae, utilizing the Yeast Gal1 Promoter and CYC100 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1705
    Illegal XbaI site found at 542
    Illegal XbaI site found at 899
    Illegal XbaI site found at 1367
    Illegal XbaI site found at 1685
    Illegal XbaI site found at 1823
    Illegal XbaI site found at 2093
    Illegal XbaI site found at 2546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1705
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1705
    Illegal BglII site found at 830
    Illegal BglII site found at 1004
    Illegal BglII site found at 1733
    Illegal BglII site found at 3005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1705
    Illegal XbaI site found at 542
    Illegal XbaI site found at 899
    Illegal XbaI site found at 1367
    Illegal XbaI site found at 1685
    Illegal XbaI site found at 1823
    Illegal XbaI site found at 2093
    Illegal XbaI site found at 2546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1705
    Illegal XbaI site found at 542
    Illegal XbaI site found at 899
    Illegal XbaI site found at 1367
    Illegal XbaI site found at 1685
    Illegal XbaI site found at 1823
    Illegal XbaI site found at 2093
    Illegal XbaI site found at 2546
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None